Skip to main content

Mgr. Alexey Bondar, Ph.D.

  • Unit:
    PřF Department of Molecular Biology and Genetics | Assistant Professor
  • E-mail:
    This email address is being protected from spambots. You need JavaScript enabled to view it.
  • Phone number:
  • Room:

Projects

Projekty lorem ipsum:

  • lorem ipsum
  • lorem ipsum
  • lorem ipsum

Publication

Detail Authors Publication title Year Publication type Publication sub-type

CV

Alexey Bondar, Ph.D.

E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. & This email address is being protected from spambots. You need JavaScript enabled to view it.

Born 15/7/1984, in Minsk, Belarus

 

Education:

2014                Ph.D. degree in Biophysics at the Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech Republic; thesis advisor: J. Lazar, Ph.D. Thesis title: “Studies of membrane protein structure and function using polarization microscopy”. Graduated with honors.

2006                Master’s degree in Biochemistry at the Belarusian State University in Minsk, Belarus; thesis advisor: M. Sholukh, Ph.D. Master’s thesis title: “Studies of the cytoprotective properties of prostaglandin groups A and E”. Graduated with honors.

2005                Bachelor’s degree in Biology at the Belarusian State University in Minsk, Belarus.

 

Employment History:

2023-present

 

 

2020-2022

 

2008-2022

 

 

2019-2022

 

2017-2019

 

2015-2017

Scientific officer (joint appointment) Laboratory of Microscopy and Histology, Institute of Entomology, Biology Centre CAS & Department of Molecular Biology and Genetics, Faculty of Science, University of South Bohemia in Ceske Budejovice, Czech Republic – microscopy facility management, maintenance, expert assistance to users

Research fellow (part time), Faculty of Science, University of South Bohemia in Ceske Budejovice, Czech Republic – studies of G protein signaling mechanisms in live cells

Research fellow, Laboratory of Structural Biology and Bioinformatics, Institute of Microbiology, Academy of Sciences, Czech Republic – development of single-molecule microscopy techniques and their application to cell signaling

Research fellow (part time), Institute of Organic Chemistry and Biochemistry, Academy of Sciences, Czech Republic – development of sensors for polarization microscopy

Postdoctoral fellow, Augusta University, Augusta, GA, USA – single-molecule studies of G protein signaling dynamics

Research fellow (part time), Institute of Organic Chemistry and Biochemistry, Academy of Sciences, Czech Republic – development of microscopy techniques

2014 (6 months)

 

2013 (6 months)

Postdoctoral fellow, Tampere University of Technology, Finland – computational studies of G protein interactions with lipid membrane components

Visiting Research Fellow, Tampere University of Technology, Finland – computational studies of G protein interactions with G protein-coupled receptors

2008-2013

Research Assistant (part time), Faculty of Science, University of South Bohemia, Czech Republic – development of imaging techniques for studies of membrane proteins

2006-2007

Research Assistant (part time), Belarusian State University, Belarus – biochemical studies of antioxidant properties of synthetic prostaglandin analogs

 

Awards and Fellowships (selected):

2022                 US and EU Patent application 18/057,629 (co-inventor with J. Lazar): Probe and method for detecting membrane-associated proteins in living cells

2020-2023        INTER-COST grant “Studies of GPCR and G-protein localization in membrane domains using single-molecule imaging”

2020-2022   Junior GAČR grant “Role of receptor/G protein preassembly in G protein signaling”

2017-2019        Junior GAČR grant “Insights into mechanisms of G protein signaling by the β2-adrenergic receptor”

2016                 Keystone “Future of Science” scholarship to attend the conference “G Protein-Coupled Receptors: Structure, Signaling and Drug Discovery”, Keystone CO, USA.

2015                 Award of the Czechoslovak Microscopy society for the best dissertation involving utilization of microscopy techniques

2014                 Award of the University of South Bohemia for outstanding scientific contribution in dissertation

2014                 Keystone “Future of Science” scholarship to attend the conference “G protein-coupled receptors: structural dynamics and functional implications”, Snowbird UT, USA.

2012                 FEBS Youth Travel Fund grant to attend the workshop “Dynamics of cell signal systems workshop”, Oslo, Norway

2010                 FEBS Youth Travel Fund grant to attend the workshop “Spatial and temporal regulation of signaling”, Oslo, Norway

2009                 EMBO travel fellowship to attend the course “Advanced optical microscopy”, UK.

2009                 FEBS Youth Travel Fund grant to attend the course “Practical course in computational biology”, Split, Croatia.

2008                 FEBS Youth Travel Fund grant to attend the course “Microspectroscopy: monitoring molecular interactions in living cells”, Wageningen, Holland.

2008                 Student research grant of the Grant Agency of the University of South Bohemia

 

Publications (Total number of citations (WOS): 197; H-index 7):

  1. Petelak A., Lambert N.A., Bondar A. 5-HT7 receptor slows down the Gs protein: a single molecule perspective Molecular Biology of the Cell (2023) [IF 3.3, cited 0x]
  2. Bondar A., Rybakova O., Melcr J., Dohnalek J., Khoroshyy P., Tichacek O., Timr S., Miclea P., Sakhi A., Markova V., Lazar J. Quantitative Linear Dichroism Imaging of Molecular Processes in Living Cells Made Simple by Open Software Tools. Communications Biology (2021) [IF 6.3, cited 1x]
  3. Bondar A.*, Lazar J. Optical sensors of heterotrimeric G protein signaling. FEBS Journal (2021) [IF 5.5, cited 5x] (*corresponding author)
  4. Myskova J., Rybakova O., Brynda J., Khoroshyy P., Bondar A., Lazar J. Directionality of light absorption and emission in representative fluorescent proteins. PNAS (2020) [IF 11.2, cited 15x]
  5. Bondar A.*, Jang W., Sviridova E., Lambert N.A. Components of the Gssignaling cascade exhibit distinct changes in mobility and membrane domain localization upon β2‐adrenergic receptor activation. Traffic (2020) [IF 6.2, cited 4x] (*co-corresponding author)
  6. Bondar A.*, Lazar J. The G protein Gi1 exhibits basal coupling but not preassembly with G protein-coupled receptors. Journal of Biological Chemistry (2017) [IF 4.0, cited 9x] (*corresponding author)
  7. Sviridova E., Rezacova P., Bondar A., Veverka V., Novak P., Schenk G., Svergun D., Kuta Smatanova I., Bumba L. Structural basis of the interaction between the putative adhesion-involved and iron-regulated FrpD and FrpC proteins of Neisseria meningitidis. Scientific Reports (2017). [IF 4.1, cited 6x]
  8. Timr S., Brabec J., Bondar A., Ryba T., Zelezny M., Lazar J., Jungwirth P. Nonlinear Optical Properties of Fluorescent Dyes Allow for Accurate Determination of Their Molecular Orientations in Phospholipid Membranes. Journal of Physical Chemistry B (2015). [IF 3.3, cited 10x]
  9. Han Z., Jin L., Chen F., Loturco J.J., Cohen L.B., Bondar A., Lazar J., Pieribone V.A. Mechanistic Studies of the Genetically Encoded Fluorescent Protein Voltage Probe ArcLight. PLoS ONE (2014). [IF 3.5, cited 38x]
  10. Bondar A.*, Lazar J. Dissociated GaGTP and Gbg protein subunits are the major activated form of heterotrimeric Gi/o proteins. Journal of Biological Chemistry (2014). [IF 4.6, cited 17x] (*corresponding author)
  11. Timr S., Bondar A., Cwiklik L., Stefl M., Hof M., Vazdar M., Lazar J. and Jungwirth P. Accurate Determination of the Orientational Distribution of a Fluorescent Molecule in a Phospholipid Membrane. Journal of Physical Chemistry B (2014) [IF 3.4, cited 22x]
  12. Lazar J., Bondar A., Timr S., Firestein S., Two-photon polarization microscopy reveals protein structure and function. Nature Methods (2011). [IF 20.7, cited 65x]
  13. Hubich , Bondar A., Kastsiuk T., Kastsiuk U., Lakhvich F., Sholukh M., Hepatoprotective action of prostaglandin A2 analogs under CCl4-induced liver injury in vitro. Hepatology Research (2007). [IF 1.892, cited 6x]

 

Conference Presentations (selected):

2020      Bondar A. Preassembly between the G protein-coupled receptors and G proteins studied at the single-molecule level // 3rd COST ERNEST meeting (Invited talk)

2018      Bondar A., Lambert N.A. G proteins avoid, but are not excluded from, clathrin-coated structures // Gordon Research Conference Phosphorylation and G-Protein Mediated Signaling Networks, Biddeford, ME, USA (Oral presentation)

2016      Bondar A., Brzakova A., Timr S., Lazar J. Precoupling of G proteins with GPCRs visualized by two-photon polarization microscopy // 60th Annual meeting of the Biophysical Society, Los Angeles CA, USA (Oral presentation)

2016      Bondar A., Lazar J. Studies of G proteins preassembly with GPCRs with two-photon polarization microscopy // Keystone Symposium G Protein-Coupled Receptors: Structure, Signaling and Drug Discovery, Keystone CO, USA (Oral presentation)

2015      Bondar A. Studies of protein structure and function using polarization microscopy // Annual meeting of Czechoslovak Microscopy Society, Lednice, Czech Republic (Invited talk)

2014      Bondar A., Lazar J. In vivo imaging and quantitation of interactions between Gi/o protein subunits and GPCRs by two-photon polarization microscopy // Keystone Symposium G protein-coupled receptors: structural dynamics and functional implications, Snowbird UT, USA (Oral presentation)

 

Tutoring of students:

2009-2019        Annual Summer School in Molecular Biophysics and Systems Biology, Nové Hrady, Czech Republic (Project leader: one-month project, 2 students/year)

2021-2022        Bachelor’s students of the joint Biochemistry program of the Johannes Kepler University in Linz and the University of South Bohemia in České Budějovice (2 students)

2022-2023        Bachelor’s student of the Faculty of Science (1 student)

 

Scientific Interests:

- Mechanisms of GPCR/G protein signal transduction

- Imaging and computational techniques for studies of membrane proteins

- Development of optical sensors for cell signaling processes

 

Member of:

COST Action ERNEST – Management Committee member, Workgroup 4 Co-Leader.

American Society for Neuroscience

American Society for Biochemistry and Molecular Biology

Biophysical society

Czech Society for Biochemistry and Molecular Biology (FEBS constituent society)

Czechoslovak Microscopy Society (EMS constituent society)

 

Languages Spoken:

Fluent: Russian (native), English (advanced), Czech (intermediate); other: Belarusian, German

Creative activity

Current schedule



Information

Budweis Single-Molecule Group

Our research group is taking the advantage of living on the border between biology and physics. We are mostly focused on single-molecule research and we are currently looking for motivated students of all degrees!

Right now we are looking for motivated students of all levels (Bc, Mgr, PhD)!

Contact information

This email address is being protected from spambots. You need JavaScript enabled to view it.

This email address is being protected from spambots. You need JavaScript enabled to view it.This email address is being protected from spambots. You need JavaScript enabled to view it.

Address:
Building C (room 01037) 
Faculty of Science
University of South Bohemia in České Budějovice
Branišovská 1760
370 05 České Budějovice


Phone: 00420387776237
GPS: 48.9772847N, 14.4451364E

Studies of G protein signaling at the single molecule level

- Getting insights into non-canonical G protein signaling and development of signaling activity sensors

Motivation:

The G protein signaling cascade is a major pathway responsible for cellular communication with the external environment. It is present in all eukaryotes from yeast to humans. G proteins transduce signals from a variety of chemical and physical stimuli including hormones, odorants, neurotransmitters, and light. Up to 50% of all modern prescription drugs target this signaling cascade. However, many aspects of G protein signaling remain unclear. Our goal is to gain insights into non-conventional properties of G protein signaling in mammalian and fungal cells. Particularly, we are interested in precoupling between G proteins and G protein-coupled receptors (GPCRs), dimerization and heteromerization of GPCSs, and development of signaling activity sensors (1-5). In our research we use advanced imaging techniques, including single-molecule imaging, combined with molecular biology, cell biology methods and advanced data processing. We strive to utilize cutting-edge techniques and multidisciplinary approaches to do excellent science.


Publications:

  1. Petelak A., Lambert N.A., Bondar A. 5-HT7 receptor slows down the Gs protein: a single molecule perspective Molecular Biology of the Cell (2023)
  2. A. Bondar, J. Lazar, Optical sensors of heterotrimeric G protein signaling. FEBS J 288, 2570-2584 (2021).
  3. A. Bondar, O. Rybakova, J. Melcr, J. Dohnalek, P. Khoroshyy, O. Tichacek, S. Timr, P. Miclea, A. Sakhi, V. Markova, J. Lazar  Quantitative Linear Dichroism Imaging of Molecular Processes in Living Cells Made Simple by Open Software Tools Communications Biology 4, 189 (2021).
  4. A. Bondar, W. Jang, E. Sviridova, N. A. Lambert, Components of the Gs signaling cascade exhibit distinct changes in mobility and membrane domain localization upon beta2-adrenergic receptor activation. Traffic 21, 324-332 (2020).
  5. A. Bondar, J. Lazar, The G protein Gi1 exhibits basal coupling but not preassembly with G protein-coupled receptors. J Biol Chem 292, 9690-9698 (2017).
  6. A. Bondar, J. Lazar, Dissociated GalphaGTP and Gbetagamma protein subunits are the major activated form of heterotrimeric Gi/o proteins. J Biol Chem 289, 1271-1281 (2014).

Subscribe
to our newsletter

Stay in touch
social media

© 2021 University of South Bohemia
Cookies

Dear visitors, we have just launched the new website of the University including all faculties, departments and constituent parts. You can still access the old version before you get used to the new website.

1