The aim of this thesis was to investigate the interaction of the salivary gland protein, Salp15, in relation to the outer surface protein C, OspC, using in silico analysis, as well as trying to amplify Salp15 from different tick species found in same geographical areas and observing the growth of Borrelia in the midgut and salivary glands of these different tick species in vitro.
Anotace v angličtině
The aim of this thesis was to investigate the interaction of the salivary gland protein, Salp15, in relation to the outer surface protein C, OspC, using in silico analysis, as well as trying to amplify Salp15 from different tick species found in same geographical areas and observing the growth of Borrelia in the midgut and salivary glands of these different tick species in vitro.
The aim of this thesis was to investigate the interaction of the salivary gland protein, Salp15, in relation to the outer surface protein C, OspC, using in silico analysis, as well as trying to amplify Salp15 from different tick species found in same geographical areas and observing the growth of Borrelia in the midgut and salivary glands of these different tick species in vitro.
Anotace v angličtině
The aim of this thesis was to investigate the interaction of the salivary gland protein, Salp15, in relation to the outer surface protein C, OspC, using in silico analysis, as well as trying to amplify Salp15 from different tick species found in same geographical areas and observing the growth of Borrelia in the midgut and salivary glands of these different tick species in vitro.
Defence was online, the student and the members of the commission were connected via MS Teams.
Prof. Grubhoffer welcomed the student and the commission.
The student presented her work „The interaction of Borrelia Outer surface proteins with tick salivary proteins“, introduction to the topic, methods, results and summary.
Supervisor Dr. Rego presented his review.
Oponnent MSc. Mahmood presented his review.
Student answered opponent´s questions: compostion of Borrelia antibiotics in the experiments, reason for using antibiotics, isolation of plasmid DNA – why white E.coli colonies, degenerate primer, primers for Salp 15, phylogenetic trees of OspC and Salp 15.
Opponent presented his comments on the thesis.
Student answered questions of the members of the commission:
Prof. Grubhoffer –
Dr. Štěrbová –
Dr. Věchtová –
Dr. Štěrba – the units of the numbers shown in the presentation