Informace o kvalifikační práci Analysis of genetic diversity of <i>Borrelia burgdorferi</i> sensu lato and <i>Borrelia miyamotoi</i> across Central and Eastern Europe using multilocus sequence typing (MLST) in range of Slovakia and data from the MLST database with focus on Central and Eastern Europe
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Hlavní téma
Analysis of genetics diversity of Borrelia burgdorferi sensu lato and Borrelia miyamotoi using multilocus sequence typing (MLST) in range of slovakia and data from the MLST database with a focus on Central and Eastern Europe
Hlavní téma v angličtině
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Název dle studenta
Analysis of genetic diversity of Borrelia burgdorferi sensu lato and Borrelia miyamotoi across Central and Eastern Europe using multilocus sequence typing (MLST) in range of Slovakia and data from the MLST database with focus on Central and Eastern Europe
Název dle studenta v angličtině
Analysis of genetic diversity of Borrelia burgdorferi sensu lato and Borrelia miyamotoi across Central and Eastern Europe using multilocus sequence typing (MLST) in range of Slovakia and data from the MLST database with focus on Central and Eastern Europe
The study was based on tick collection, DNA extraction, PCR amplification, sequencing and computational analyses of Borrelia burgdorferi sensu lato and B. miyamotoi. The study included evaluation on the prevalence of Borrelia burgdorferi sensu lato and B. miyamotoi in Ixodes ricinus ticks across the Slovak Republic based on the collection of ticks from 2017. The spirochetes were further classified into genospecies as well as sequence types (ST) according to MLST method by amplifying and sequencing eight housekeeping genes. Subsequently, population-genetic relationships of Borrelia tick-borne pathogens across Central and Eastern Europe were analysed.
Anotace v angličtině
The study was based on tick collection, DNA extraction, PCR amplification, sequencing and computational analyses of Borrelia burgdorferi sensu lato and B. miyamotoi. The study included evaluation on the prevalence of Borrelia burgdorferi sensu lato and B. miyamotoi in Ixodes ricinus ticks across the Slovak Republic based on the collection of ticks from 2017. The spirochetes were further classified into genospecies as well as sequence types (ST) according to MLST method by amplifying and sequencing eight housekeeping genes. Subsequently, population-genetic relationships of Borrelia tick-borne pathogens across Central and Eastern Europe were analysed.
The study was based on tick collection, DNA extraction, PCR amplification, sequencing and computational analyses of Borrelia burgdorferi sensu lato and B. miyamotoi. The study included evaluation on the prevalence of Borrelia burgdorferi sensu lato and B. miyamotoi in Ixodes ricinus ticks across the Slovak Republic based on the collection of ticks from 2017. The spirochetes were further classified into genospecies as well as sequence types (ST) according to MLST method by amplifying and sequencing eight housekeeping genes. Subsequently, population-genetic relationships of Borrelia tick-borne pathogens across Central and Eastern Europe were analysed.
Anotace v angličtině
The study was based on tick collection, DNA extraction, PCR amplification, sequencing and computational analyses of Borrelia burgdorferi sensu lato and B. miyamotoi. The study included evaluation on the prevalence of Borrelia burgdorferi sensu lato and B. miyamotoi in Ixodes ricinus ticks across the Slovak Republic based on the collection of ticks from 2017. The spirochetes were further classified into genospecies as well as sequence types (ST) according to MLST method by amplifying and sequencing eight housekeeping genes. Subsequently, population-genetic relationships of Borrelia tick-borne pathogens across Central and Eastern Europe were analysed.
10 Appendices - schemes of population-genetic analyses of individual genospecies of Borrelia burgdorferi sensu lato
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Převzato z knihovny
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Plný text práce
Přílohy
Posudek(y) oponenta
Hodnocení vedoucího
Záznam průběhu obhajoby
Uchazečka seznámila komisi s výsledky své diplomové práce. Školitel a oponenti přečetli své posudky. Posudky oponentů obashovaly řadu výhrad k metodickým stránkám práce a jejich prezentaci v textu. Část otázek se týkala vlastní biologie borelií. Uchazečka odpověděla na vznesené dotazy (část dotazů byla předem zodpovězena prostřednictvím emailu a komise byla seznámena s odpověďmi). Následovala diskuse na téma některých metodických postupů a interpretace výsledků. Samostatnou otázkou byl podíl uchazečky na získání předkládaných dat a výsledků. V uzavřeném jednání komise konstatovala, že není zcela zřejmé, zda uchazečka prezentovala data a výsledky v souladu se všemi etickými a formálními pravidly (tedy zda je oprávněna disponovat daty zahrnutými v práci). Komise hlasovala v tajném hlasování pouze k otázce kvality práce a obhajoby. Otázka souladu předložené práce s pravidly bude řešena dodatečně.