Aims of this thesis were to annotate novel transcripts within clusters of imprinted genes in mouse oocytes and embryo, to analyze expression changes of these transcripts during mouse embryonic development, to identify enriched sequence motifs and potential transcription factors binding sites at promoters of transcripts within imprinted gene clusters as well as transposable elements acting as promoters of these transcripts and to identify potential candidates for further functional studies, using bioinformatic methods.
Anotace v angličtině
Aims of this thesis were to annotate novel transcripts within clusters of imprinted genes in mouse oocytes and embryo, to analyze expression changes of these transcripts during mouse embryonic development, to identify enriched sequence motifs and potential transcription factors binding sites at promoters of transcripts within imprinted gene clusters as well as transposable elements acting as promoters of these transcripts and to identify potential candidates for further functional studies, using bioinformatic methods.
Klíčová slova
genetics, bioinformatics, imprinting in mammals, novel trananscripts, expression analysis, imprinted genes, mouse transcriptome, transcription factors, regulation of imprinting, transposable elements
Klíčová slova v angličtině
genetics, bioinformatics, imprinting in mammals, novel trananscripts, expression analysis, imprinted genes, mouse transcriptome, transcription factors, regulation of imprinting, transposable elements
Rozsah průvodní práce
68 p.
Jazyk
AN
Anotace
Aims of this thesis were to annotate novel transcripts within clusters of imprinted genes in mouse oocytes and embryo, to analyze expression changes of these transcripts during mouse embryonic development, to identify enriched sequence motifs and potential transcription factors binding sites at promoters of transcripts within imprinted gene clusters as well as transposable elements acting as promoters of these transcripts and to identify potential candidates for further functional studies, using bioinformatic methods.
Anotace v angličtině
Aims of this thesis were to annotate novel transcripts within clusters of imprinted genes in mouse oocytes and embryo, to analyze expression changes of these transcripts during mouse embryonic development, to identify enriched sequence motifs and potential transcription factors binding sites at promoters of transcripts within imprinted gene clusters as well as transposable elements acting as promoters of these transcripts and to identify potential candidates for further functional studies, using bioinformatic methods.
Klíčová slova
genetics, bioinformatics, imprinting in mammals, novel trananscripts, expression analysis, imprinted genes, mouse transcriptome, transcription factors, regulation of imprinting, transposable elements
Klíčová slova v angličtině
genetics, bioinformatics, imprinting in mammals, novel trananscripts, expression analysis, imprinted genes, mouse transcriptome, transcription factors, regulation of imprinting, transposable elements